Publications &
Book chapters

Free-living amoebae

  • Delumeau A, Quétel I, Harnais F, Sellin A, Gros O, Talarmin A, Marcelino I. (2023) Bacterial microbiota management in free-living amoebae (Heterolobosea lineage) isolated from water: The impact of amoebae identity, grazing conditions, and passage number. The Science of the total environment, doi: 10.1016/j.scitotenv.2023.165816

  • Dereeper A, Allouch N, Guerlais V, Garnier M, Ma L, De Jonckheere JF, Joseph SJ, Ali IK, Talarmin A, Marcelino I. (2022) Naegleria genus pangenome reveals new structural and functional insights into the versatility of these free-living amoebae. Frontiers in Microbiology, doi: 10.3389/fmicb.2022.1056418

  • Reynaud Y, Ducat C, Talarmin A, Marcelino I. (2020) Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding. Pathogens, doi: 10.3390/pathogens9060440.

  • Moussa M, Marcelino I, Richard V, Guerlotté J, Talarmin A. (2020) An Optimized Most Probable Number (MPN) Method to Assess the Number of Thermophilic Free-Living Amoebae (FLA) in Water Samples. Pathogens, doi:10.3390/pathogens9050409.

  • Moussa M, Tissot O, Guerlotté J, De Jonckheere JF, Talarmin A. Soil is the origin for the presence of Naegleria fowleri in the thermal recreational waters. Parasitol Res. 2015 Jan;114(1):311-5. doi: 10.1007/s00436-014-4197-x.

  • Moussa M, De Jonckheere JF, Guerlotté J, Richard V, Bastaraud A, Romana M, Talarmin A. Survey of Naegleria fowleri in geothermal recreational waters of Guadeloupe (French West Indies). PLoS One. 2013;8(1):e54414. doi: 10.1371/journal.pone.0054414.

E.ruminantium pathogenesis

  • Pinarello V, Bencurova E, Marcelino I, Gros O, Puech C, Bhide M, Vachiery N, Meyer DF. (2021) Ehrlichia ruminantium uses its transmembrane protein Ape to adhere to host bovine aortic endothelial cells. bioRxiv, doi:10.1101/2021.06.15.447525.

  • Marcelino I, Holzmuller P, Coelho A, Mazzucchelli G, Fernandez B, Vachiéry N. (2021) Revisiting Ehrlichia ruminantium Replication Cycle Using Proteomics: The Host and the Bacterium Perspectives. Microorganisms, doi: 10.3390/microorganisms9061144.

  • Marcelino I, Colomé-Calls N, Holzmuller P, Lisacek F, Reynaud Y, Canals F, Vachiéry N. (2019) Sweet and Sour Ehrlichia: Glycoproteomics and Phosphoproteomics Reveal New Players in Ehrlichia ruminantium Physiology and Pathogenesis. Front Microbiol, doi: 10.3389/fmicb.2019.00450.

  • Marcelino I, Holzmuller P, Stachurski F, Rodrigues V, Vachiéry N. (2016) Ehrlichia ruminantium: The Causal Agent of Heartwater. In: Thomas S. (eds) Rickettsiales. Springer, Cham.

  • Marcelino I, Ventosa M, Pires E, Müller M, Lisacek F, Lefrançois T, Vachiery N, Coelho AV. (2015) Comparative Proteomic Profiling of Ehrlichia ruminantium Pathogenic Strain and Its High-Passaged Attenuated Strain Reveals Virulence and Attenuation-Associated Proteins. PLoS One, doi: 10.1371/journal.pone.0145328.

  • Moumène A, Marcelino I, Ventosa M, Gros O, Lefrançois T, Vachiéry N, Meyer DF, Coelho AV. (2015) Proteomic profiling of the outer membrane fraction of the obligate intracellular bacterial pathogen Ehrlichia ruminantium. PLoS One, doi: 10.1371/journal.pone.0116758.

  • Pruneau L, Moumène A, Meyer DF, Marcelino I, Lefrançois T, Vachiéry N. (2014) Understanding Anaplasmataceae pathogenesis using "Omics" approaches. Front Cell Infect Microbiol, doi: 10.3389/fcimb.2014.00086.

  • Vachiéry N, Marcelino I, Martinez D, Lefrançois T. (2014) Manual of Security Sensitive Microbes and Toxins. Chapter 65 - Ehrlichia ruminantium. In book: Manual of Security Sensitive Microbes and ToxinsChapter: Ehlichia ruminantium. Publisher: Taylor & Francis Group, LLC, Editors: Dongyou Liu, DOI: 10.1201/b16752-72

  • Marcelino I, Ventosa M, Pruneau L, Pires E, Meyer DF, de Almeida AM, Mari B, Lefrançois T, Coelho AV, Vachiéry N. (2013) Omics approaches to study the Rickettsia Ehrlichia ruminantium: towards improved knowledge on Heartwater disease. In: de Almeida A. et al. (eds) Farm animal proteomics 2013. Wageningen Academic Publishers, Wageningen. Doi: 10.3920/978-90-8686-776-9_30.

  • Ventosa M, Marcelino I, Coelho AV, Horlacher O, Lisacek F, Vachiéry N, Lefrançois T (2013) Automatic prediction of PTMs in Ehrlichia ruminantium – creating new datasets for Quickmod analyses. In: de Almeida A. et al. (eds) Farm animal proteomics 2013. Wageningen Academic Publishers, Wageningen. Doi: 10.3920/978-90-8686-776-9_20.

  • Pires E, Marcelino I, Vachiéry N, Lefrançois T, Mazzuchelli G, de Pauw E, Coelho AV. (2013) Changes on bovine aorta endothelial cells (BAE) proteome upon infection with the rickettsia Ehrlichia ruminantium. In: de Almeida A. et al. (eds) Farm animal proteomics 2013. Wageningen Academic Publishers, Wageningen. Doi: 10.3920/978-90-8686-776-9_33

  • Marcelino I, de Almeida AM, Ventosa M, Pruneau L, Meyer DF, Martinez D, Lefrançois T, Vachiéry N, Coelho AV. (2012) Tick-borne diseases in cattle: applications of proteomics to develop new generation vaccines. J Proteomics, doi: 10.1016/j.jprot.2012.03.026.

  • Marcelino I, de Almeida AM, Brito C, Meyer DF, Barreto M, Sheikboudou C, Franco CF, Martinez D, Lefrançois T, Vachiéry N, Carrondo MJ, Coelho AV, Alves PM. (2012) Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins. Vet Microbiol, doi: 10.1016/j.vetmic.2011.11.022.

  • Pruneau L, Emboulé L, Gely P, Marcelino I, Mari B, Pinarello V, Sheikboudou C, Martinez D, Daigle F, Lefrançois T, Meyer DF, Vachiery N. (2012) Global gene expression profiling of Ehrlichia ruminantium at different stages of development. FEMS Immunol Med Microbiol, doi: 10.1111/j.1574-695X.2011.00901.x.

Inactivated vaccine production

  • Marcelino I, Chavez A, Gharbi M, Farber M, Holzmuller P, Martinez D, Vachiéry N. Protozoal and Rickettsial Vaccines. In book: Veterinary Vaccines: Principles and Applications, John Wiley & Sons, 2021. Doi: 10.1002/9781119506287.ch7

  • Marcelino I, Lefrançois T, Martinez D, Giraud-Girard K, Aprelon R, Mandonnet N, Gaucheron J, Bertrand F, Vachiéry N. (2015) A user-friendly and scalable process to prepare a ready-to-use inactivated vaccine: the example of heartwater in ruminants under tropical conditions. Vaccine, doi: 10.1016/j.vaccine.2014.11.

  • Vachiéry N, Marcelino I, Martinez D, Lefrançois T. (2013) Opportunities in diagnostic and vaccine approaches to mitigate potential heartwater spreading and impact on the American mainland. Dev Biol (Basel), doi: 10.1159/000190050.

  • Vachiéry N, Meyer D, Marcelino I, Alves PM, Raliniaina M, Stachurski F, Adakal H, Sheikboudou C, Aprelon R, Pinarello V, Lefrançois, Dominique Martinez. (2010). Vaccinal approach using inactivated vaccine against heartwater and Ehrlichia ruminantium genetic diversity. Advances in Animal Biosciences, doi: 10.1017/S2040470010000178.

  • Adakal H, Stachurski F, Konkobo M, Zoungrana S, Meyer DF, Pinarello V, Aprelon R, Marcelino I, Alves PM, Martinez D, Lefrancois T, Vachiéry N. (2010). Efficiency of inactivated vaccines against heartwater in Burkina Faso: impact of Ehrlichia ruminantium genetic diversity. Vaccine, doi: 10.1016/j.vaccine.2010.04.087.

  • Marcelino I, Sousa MFQ, Peixoto C, Amaral AI, Vachiery N, Lefrançois T, Martinez D, Carrondo MJTC, Alves PM. (2010) Development of a Vaccine Candidate Against Heartwater. In: Noll T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. Doi: 10.1007/978-90-481-3419-9_122

  • Faburay B, Geysen D, Ceesay A, Marcelino I, Alves PM, Taoufik A, Postigo M, Bell-Sakyi L, Jongejan F. (2007) Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines. Vaccine, doi: 10.1016/j.vaccine.2007.09.002.

  • Marcelino I, Vachiéry N, Amaral AI, Roldão A, Lefrançois T, Carrondo MJ, Alves PM, Martinez D. (2007) Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater. Vaccine, doi: 10.1016/j.vaccine.2007.04.055.

  • Peixoto C, Marcelino I, Amaral AI, Carrondo MJT, Alves PM. (2007) Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater. Process Biochemistry, doi: 10.1016/j.procbio.2007.04.012

  • Marcelino I, Sousa MFQ, Amaral AI, Peixoto C, Verissimo C, Cunha A, Carrondo MJT, Alves PM. (2007) Process Development for a Veterinary Vaccine Against Heartwater Using Stirred Tanks. In: Smith R. (eds) Cell Technology for Cell Products. Springer, Dordrecht. Doi: 10.1007/978-1-4020-5476-1_116

  • Marcelino I, Sousa MFQ, Veríssimo C, Cunha AE, Carrondo MJ, Alves PM (2006). Process development for the mass production of Ehrlichia ruminantium. Vaccine, doi: 10.1016/j.vaccine.2005.08.109.

  • Marcelino I, Veríssimo C, Sousa MF, Carrondo MJ, Alves PM. (2005) Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures. Vet Microbiol, doi: 10.1016/j.vetmic.2005.07.012. PMID: 16139967.

  • Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM. (2005) Quantification of Ehrlichia ruminantium by real time PCR. Vet Microbiol, doi: 10.1016/j.vetmic.2005.02.001.

  • Moreira JL, Miranda PM, Marcelino I, Alves PM, Carrondo MJT. (2003) Culture Methods for Mass Production of Ruminant Endothelial Cells. Fermentation Biotechnology, doi:10.1021/bk-2003-0862.ch008

Others research topics:

  • Matias AA, Serra AT, Silva AC, Perdigão R, Ferreira TB, Marcelino I, Silva S, Coelho AV, Alves PM, Duarte CM. (2010) Portuguese winemaking residues as a potential source of natural anti-adenoviral agents. Int J Food Sci Nutr, doi: 10.3109/09637480903430990.

  • Carvalhal AV, Marcelino I, Carrondo MJ. (2003) Metabolic changes during cell growth inhibition by p27 overexpression. Appl Microbiol Biotechnol, doi: 10.1007/s00253-003-1385-5.

  • Carvalhal AV, Santos SS, Coroadinha A, Marcelino I, Carrondo MJT. (2003) Cell Growth Arrest by Nucleotides as a Tool for Improved Production of Biopharmaceuticals. In: Yagasaki K., Miura Y., Hatori M., Nomura Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. Doi: 10.1007/978-94-017-0726-8_14.